Journal: Protein expression and purification
Article Title: Modular cell line for scalable and rapid in vitro evaluation of chimeric antigen receptors.
doi: 10.1016/j.pep.2025.106738
Figure Lengend Snippet: Fig. 3. Schematic representation of pWPXL-aNab-Short and pWPXL-aNab-Long vectors (a); alfa-GFP stained cells (b); Flow cytometry analysis of transduced K-562 cells (c) before and (d) after selection step with alfa-GFP staining; The dynamics of alfa-GFP signal during cultivation of K-562, K-562-Short, and K-562-Long cells (f); The dynamics of MFI values during incubation for K-562-Long or K-562-Short cells (e).
Article Snippet: The assembly of constructs was performed in the pWPXL lentiviral vector (#12257, Addgene) under the constant EF-1a promoter, followed by the components described previously.
Techniques: Staining, Flow Cytometry, Selection, Incubation